blomia tropicalis wikipedia

Blomia tropicalis allergens are the most important mite allergens in tropical regions. Their body parts and fecal excreta are initially 10 to 50 mm in diameter but break down into smaller fragments that become airborne when dust is disturbed. This finding corroborated the work of Takeda and collaborators [15], who found an increase of total IgE in BtE- and cholera toxin- sensitized mice. Health issues Allergies . Eight to 10 week-old CBA/J, BALB/c, A/J and C57BL/6 male mice, and 3 to 4 month-old Wistar rats, were bred and maintained at the animal houses of the Gonçalo Moniz Research Center, Oswaldo Cruz Foundation, Salvador, Brazil, and of the Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil. Rizzo MC, Fernandez-Caldas E, Sole D, Naspitz CK: IgE antibodies to aeroallergens in allergic children in Sao Paulo, Brazil. Geographical distribution See common geographical background to mites in our Scientific Documents (link to the right). The Blomia tropicalis storage mite belongs to the Glycyphagidae family. All used batches contained 30-40 ng of this allergen per μg of protein. TB conducted the majority of the experiments involving different mouse strains, the OVA × BtE comparison experiments and wrote the first manuscript draft. CAS  PubMed  Serum anti-OVA or anti-BtE IgG1 and IgG2a antibodies were measured using OVA- or BtE-coated microtitre plates and biotin-conjugated anti-mouse IgG1 or anti-mouse IgG2a, respectively (Pharmingen, St. Diego, CA, USA), in conjunction with streptavidin-horseradish peroxidase, H2O2 and orthophenylenodiamine (Merck, Whitehouse Station, NJ, USA). BtE-sensitized mice had higher inflammation and cell influx than saline-treated control mice. Immunization of A/J mice with BtE induced a more intense airway eosinophil influx, higher levels of total IgE, similar airway hyperreactivity to methacholine but less intense mucous production, and lower levels of specific IgE, IgG1 and IgG2 antibodies than sensitization with OVA. The protein content of the aqueous extract was determined by the Folin reagent method, described by Lowry and collaborators [19], and was subsequently stored at -70°C until use. Lipids from the lysate were extracted and discarded by five or six ether extractions. Notably, mice sensitized with BtE produced higher amounts of total IgE than those sensitized with OVA, in amounts similar to those observed with immunization with helminth antigens [32]. Google Scholar. The BALF supernatants were stored at -70°C until used. Figure 1G shows that specific IgG1 was produced in all BtE-sensitized mice and that its levels were statistically different from those of the control mice (p < 0.001 for BALB/C, C57Bl/6, and A/J mice, and p < 0.05 for CBA/J; Tukey's test). The production of IL-4 in BtE-sensitized and challenged mice was higher in A/J when compared with the other studied mouse strains (Tukey's test, p < 0.05; Figure 1E); it was followed by the production in CBA/J mice (p < 0.01, Tukey's test). PBS containing 1% of bovine serum albumin. Conventional models of allergic asthma rely on the sensitization of experimental animals to ovalbumin (OVA). Previous study reported that sensitization and challenge with BtE induce a more pronounced airway accumulation of neutrophils than eosinophils [16]. Polo día, o home perde 1 g destes pedazos de pel. To compare the means of two groups, the Student's t test was used for parametric data and the Mann-Whitney's test for non-parametric data. Altogether, we concluded that the short-term experimental model of BtE-induced asthma is reproducible in different mouse strains, although the A/J mice are the best responders, and small quantities of BtE may be used to sensitize this mouse strain. Groups of mice were sensitized subcutaneously with BtE co-adsorbed into alum on days 0 and 7, challenged intranasally with BtE on days 8, 10, 12 and 14 and studied 24 h later. volume 11, Article number: 51 (2010) Presence of mucus in bronchi of A/J mice sensitized with ovalbumin (OVA),Blomia tropicalisextract (Bt) or saline (Control). This data is representative of three independent experiments. J Allergy Clin Immunol 2006, 117:283–290. (B) Eosinophil numbers in the BALF. Unreviewed (50) TrEMBL. Cite this article. Total IgE was detected using anti-mouse IgE-coated microtitre plates and biotin-conjugated anti-mouse IgE (UNLB Southern Biotechnology Associates, Inc., Birmingham, AL, USA), in conjunction with streptavidin-horseradish peroxidase, H2O2 and orthophenylenodiamine. Mice of all strains had lung inflammatory-cell infiltration and increased levels of anti-BtE IgE antibodies, but these responses were significantly more intense in A/J mice than in CBA/J, BALB/c or C57BL/6J mice. The authors declare that they have no competing interests. *p < 0.05, **p < 0.01, and ***p < 0.001 for the indicated tested groups (Tukey's test). In B and D, columns represent the mean result of 5 (B) or 8 (D) animals; the vertical bars represent the standard deviation of the means. JCI 2006,116(2):306–8. The first objective of the present work was to study the murine allergic response to B. tropicalis using a short-term immunization protocol. … A quantitative digital morphometric analysis was performed using the application program Metamorph 6.0 (Universal Images Corp. Downingtown, PA, USA). This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. *p < 0.05, **p < 0.01, ***p < 0.001. (A) Mucus Index in the bronchi. It is abbreviated Blot by the World Health Organization. P > 0.05 is not represented. Airway responsiveness to increasing doses of inhaled methacholine (3, 6, 12 and 25 mg/mL) in conscious unrestrained mice was determined using a single-chamber, whole-body plethysmograph (Buxco Electronics Inc., Wilmington, NC, USA), as previously described [21]. Format. Lab Anim Sci 1998,48(4):346–52. The tracheas of the dead mice were cannulated and the BALF collected in 0.5 mL of PBS containing 1% of bovine serum albumin (Sigma Chemical Co., St. Louis, MO, USA; PBS-BSA). Thus, sensitization to B. tropicalis allergens is considered an independent and important cause of allergy [4, 8]. Allergic response of A/J mice sensitized withBlomia tropicalisextract (Bt E) or ovalbumin (OVA) or injected with saline (Control). 072405/Z/03/Z). Cheong N, Siew CS, Ramos JDA, Kuo IC, Prasanna K, Lee BW, Chua KY. There is evidence that allergens from B. tropicalis are distinct from, and bear only low to moderate cross-reactivity to allergens from Dermatophagoides sp. Sensitization with OVA (p < 0.001, Tukey's test) and BtE (p < 0.01, Tukey's test) induced AHR, as compared with control mice (Figure 2D). After 48 hours, the rats received 2 mg of BtE in the tail vein, diluted in 0.5 mL of saline containing 0.5 mg/mL of Evans blue (Sigma Chemical Co, St. Louis, MO, USA). Tsai JJ, Wu HH, Shen HD, Hsu EL, Wang SR: Sensitization to Blomia tropicalis among asthmatic patients in Taiwan. © 2021 BioMed Central Ltd unless otherwise stated. Existem duas espécies mais prevalentes no Brasil - Dermatophagoides pteronyssinus e D. farinae.O ácaro Dermatophagoides é considerado o principal agente causador de alergia respiratória no Brasil junto com outro ácaro conhecido como Blomia tropicalis. Yu P, Kosco-Vilbois M, Richards M, Köhler G, Lamers MC: Negative feedback regulation of IgE synthesis by murine CD23. The EPO activity was higher in the BALF of BtE-sensitized mice than in that of negative controls (Figure 5C; p < 0.01; Student's t test). P > 0.05 is not represented. These mice were also compared with OVA-immunized A/J mice in all the immunological and inflammatory parameters that were mentioned above. Clin Exp Allergy 1999,29(7):982–8. J Immunol Methods 1985,83(2):209–15. After the observation that A/J was the best mouse strain for BtE-induced asthma, we immunized these mice with a low-dose (10 μg per injection) of BtE instead of the 100 μg dose per injection used in the previous experiments. Tissues sections of 5-μm were then stained with periodic acid-Schiff (PAS) for the evaluation of mucus production. Article  In brief, 0.05 mL volumes of double dilutions (1/4 to 1/512) of individual mouse serum samples were intradermically injected in the shaved dorsal regions of Wistar rats. Storage mites can also adapt their feeding to house dust. )是一種8隻腳的微小動物,屬於節肢動物門 蛛形綱 恙蟎目 塵蟎科。 是台灣兒童主要的過敏原之一 。 responses to BtE baqueiro, T., Russo, M. Silva. That inbred mouse strains using a short-term protocol using B. tropicalis is an important source of in... Allergol Clin Immunol 1987,80 ( 6 ):664–8 ) the Scientific name for a common house mite in! 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